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recombinant np protein  (Sino Biological)


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    Sino Biological recombinant np protein
    Recombinant Np Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 95/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant np protein/product/Sino Biological
    Average 95 stars, based on 48 article reviews
    recombinant np protein - by Bioz Stars, 2026-02
    95/100 stars

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    Direct ELISA data reporting the binding of 4D4 (panel A) and 1H6 (panel B) MAbs to hCoV NP. MAbs were conjugated to HRP and binding to 1 μg/mL <t>of</t> <t>recombinant</t> viral nucleocapsid protein (NP) reported with an enhanced chemiluminescent substrate (ECL) and a luminometer. Binding data is expressed as the mean ± SEM in counts per second (CPS) with BSA and no antigen (dashed red line) controls. See recombinant NP details in Table [2–9]. Key: [2] SARS‐CoV‐2 B.1.1.529 from <t>HEK</t> 293. [3] SARS‐CoV‐2 Wuhan‐Hu‐1. [4] SARS‐CoV. [5] MERS‐CoV. [6] hCoV‐OC43. [7] hCoV‐HKU1. [8] hCoV‐NL63. [9] hCoV‐229E.
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    Image Search Results


    Direct ELISA data reporting the binding of 4D4 (panel A) and 1H6 (panel B) MAbs to hCoV NP. MAbs were conjugated to HRP and binding to 1 μg/mL of recombinant viral nucleocapsid protein (NP) reported with an enhanced chemiluminescent substrate (ECL) and a luminometer. Binding data is expressed as the mean ± SEM in counts per second (CPS) with BSA and no antigen (dashed red line) controls. See recombinant NP details in Table [2–9]. Key: [2] SARS‐CoV‐2 B.1.1.529 from HEK 293. [3] SARS‐CoV‐2 Wuhan‐Hu‐1. [4] SARS‐CoV. [5] MERS‐CoV. [6] hCoV‐OC43. [7] hCoV‐HKU1. [8] hCoV‐NL63. [9] hCoV‐229E.

    Journal: Microbial Biotechnology

    Article Title: Immunoassay Detection of SARS ‐ CoV ‐2 Using Monoclonal Antibody Binding to Viral Nucleocapsid Protein

    doi: 10.1111/1751-7915.70117

    Figure Lengend Snippet: Direct ELISA data reporting the binding of 4D4 (panel A) and 1H6 (panel B) MAbs to hCoV NP. MAbs were conjugated to HRP and binding to 1 μg/mL of recombinant viral nucleocapsid protein (NP) reported with an enhanced chemiluminescent substrate (ECL) and a luminometer. Binding data is expressed as the mean ± SEM in counts per second (CPS) with BSA and no antigen (dashed red line) controls. See recombinant NP details in Table [2–9]. Key: [2] SARS‐CoV‐2 B.1.1.529 from HEK 293. [3] SARS‐CoV‐2 Wuhan‐Hu‐1. [4] SARS‐CoV. [5] MERS‐CoV. [6] hCoV‐OC43. [7] hCoV‐HKU1. [8] hCoV‐NL63. [9] hCoV‐229E.

    Article Snippet: Female Balb/cByJ mice (The Jackson Laboratory, ME) at 6 weeks of age were immunised every 10d, 4 times, by intraperitoneal inoculation with a 50 μL emulsion of 5 μg recombinant SARS‐CoV‐2 nucleocapsid protein (NP) Omicron B.1.1.529 variant from human embryonic kidney (HEK) cells (Acro Biosystems, DE) diluted in equal volume in TiterMax gold adjuvant (Sigma, MO).

    Techniques: Direct ELISA, Binding Assay, Recombinant

    Western blots showing the detection of disulfide reduced and heat denatured recombinant viral nucleocapsid proteins (NP) after electrophoresis (1 μg) with 4D4 (panel A) and 1H6 (panel B) MAb. Images were acquired at 30 s from nitrocellulose blots using a chemiluminescent substrate with HRP‐conjugated MAb (1 μg/mL) detection. A mixed standard was used to define protein migration by molecular weight (kDa). See recombinant NP details in Table [1–9]. Key: [1] SARS‐CoV‐2B 1.1.529 from E. coli . [2] SARS‐CoV‐2 B.1.1.529 from HEK 293. [3] SARS‐CoV‐2 Wuhan‐Hu‐1. [4] SARS‐CoV. [5] MERS‐CoV. [6] hCoV‐OC43. [7] hCoV‐HKU1. [8] hCoV‐NL63. [9] hCoV‐229E.

    Journal: Microbial Biotechnology

    Article Title: Immunoassay Detection of SARS ‐ CoV ‐2 Using Monoclonal Antibody Binding to Viral Nucleocapsid Protein

    doi: 10.1111/1751-7915.70117

    Figure Lengend Snippet: Western blots showing the detection of disulfide reduced and heat denatured recombinant viral nucleocapsid proteins (NP) after electrophoresis (1 μg) with 4D4 (panel A) and 1H6 (panel B) MAb. Images were acquired at 30 s from nitrocellulose blots using a chemiluminescent substrate with HRP‐conjugated MAb (1 μg/mL) detection. A mixed standard was used to define protein migration by molecular weight (kDa). See recombinant NP details in Table [1–9]. Key: [1] SARS‐CoV‐2B 1.1.529 from E. coli . [2] SARS‐CoV‐2 B.1.1.529 from HEK 293. [3] SARS‐CoV‐2 Wuhan‐Hu‐1. [4] SARS‐CoV. [5] MERS‐CoV. [6] hCoV‐OC43. [7] hCoV‐HKU1. [8] hCoV‐NL63. [9] hCoV‐229E.

    Article Snippet: Female Balb/cByJ mice (The Jackson Laboratory, ME) at 6 weeks of age were immunised every 10d, 4 times, by intraperitoneal inoculation with a 50 μL emulsion of 5 μg recombinant SARS‐CoV‐2 nucleocapsid protein (NP) Omicron B.1.1.529 variant from human embryonic kidney (HEK) cells (Acro Biosystems, DE) diluted in equal volume in TiterMax gold adjuvant (Sigma, MO).

    Techniques: Western Blot, Recombinant, Electrophoresis, Migration, Molecular Weight